TAP deficient cells

By using TAP deficient cells, we found that TAP is also necessary for the process of presentation of the antigen in MHC class I.

We further characterized the antigen depot by confocal microscopy and found that the antigen co-localized with the lysosomal marker LAMP1, but not with the endosomal marker EEA1, TAP, MHC class I or MHC class II. We conclude that the antigen depot is a storage compartment and not a loading compartment. We have started to examine depot formation by targeting antigen to other receptors. For example, we have found that presentation of TLR ligand- long peptide conjugates was also long-lived, while presentation of OVA, targeted via the mannose receptor, was not long-lived.

• molecule type
• Major histocompatibility complex class I

created over 15 years ago (15 December 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]